Related Experiment Video
Updated: Jun 22, 2026
Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
Published on: February 9, 2020
[Effect of omega-3 long-chain polyunsaturated fatty acids on the cultured oligodendrocyte precursor cells]
Xiu-jun Yu1, Bing Yu, Zhen-zhong Li
1Department of Neurology, the Second Hospital, Hebei Medical University, Shijiazhuang 050000, China. yuxiujun720309@163.com
Objective:
To explore the effect of long-chain polyunsaturated fatty acids (LPFA) on the survival and process growth of the brain hippocampal Oligodendrocyte precursor cells (OPC).
Method:
Two kinds of cultured neural progenitor cells isolated from adult rat hippocampus were used. After arachidonic acid (AA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) treatment respectively, the activity of cells were determined by Lactate dehydrogenase (LDH) assay, and the quantitative measurements of the cell processes were done after the fluorescent immune cells staining.
Results:
EPA, DHA and AA showed similar trends to increase the cell numbers when the concentrations were high (50 micromol/L, P<0.05), but only EPA and DHA elongated the process of OPC significantly (P<0.05).
Conclusion:
omega-3 LPFA (EPA and DHA) could enhance the survival and process growth of OPC.
More Related Videos
10:53Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions
Published on: November 9, 2020
11:11Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
Published on: February 17, 2016