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Updated: Jun 22, 2026

Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells
Published on: June 15, 2013
Effects of hydroxylpropyl-β-cyclodextrin on in vitro insulin stability
Liefeng Zhang1, Wenjie Zhu1, Lingling Song1
1Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, Nanjing Normal University, Nanjing 210097, P.R. China.
Abstract:
The objective of this study was to elucidate the effects of hydroxylpropyl-beta-cyclodextrin (HP-beta-CD) on the in vitro stability of insulin. It was found that HP-beta-CD had positive effects on the stability of insulin in acid and base and under high temperature conditions. Furthermore, use of HP-beta-CD could also increase the stability of disulfide bonds which are important to the conformation of insulin. Through (1)H-NMR experiments it was found that the protective effect of HP-beta-CD was due to complexation with insulin. The results suggest that the presence of HP-beta-CD could improve the stability of insulin in different environments.
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