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Updated: Jun 22, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
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Disease-specific biomarker discovery by aptamers.

Henning Ulrich1, Carsten Wrenger

  • 1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil. henning@iq.usp.br

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|July 1, 2009
PubMed
Summary

This study reviews aptamers, which are novel high-affinity molecules for cell identification. Aptamers target specific cell surface markers, aiding in distinguishing cell types and identifying disease biomarkers.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Aptamers are nucleic acid-based ligands selected through Systematic Evolution of Ligands by EXponential enrichment (SELEX).
  • They offer high specificity and affinity as capture agents, adaptable for various applications.
  • Cell SELEX utilizes whole cells as targets to identify aptamers binding to specific cell surface biomarkers.

Purpose of the Study:

  • To review the development of aptamers targeting parasite-derived surface proteins.
  • To highlight the application of aptamers in distinguishing between different cell types and identifying disease markers.
  • To discuss aptamer utility in cell identification and protein purification.

Main Methods:

  • Systematic Evolution of Ligands by EXponential enrichment (SELEX) for aptamer development.
  • Cell SELEX for targeting cell surface epitopes.
  • Application of aptamers in flow cytometry, microscopy, and ligand-mediated purification.

Main Results:

  • Aptamers have been developed that specifically bind to cell surface epitopes of trypanosomes.
  • Aptamers can distinguish between molecular signatures of somatic and cancer cells.
  • Aptamers facilitate target cell identification and purification of binding proteins.

Conclusions:

  • Aptamers represent a versatile class of molecules for specific cell targeting and biomarker discovery.
  • Cell SELEX is a powerful approach for identifying aptamers against complex cell surface targets.
  • Aptamers hold significant potential in diagnostics and therapeutics, particularly for parasitic diseases.