Related Experiment Video
Updated: Jun 22, 2026
A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
Published on: October 28, 2021
[UP-PCR diversity analysis of Fusarium population isolated from greenhouse melon soils]
Bai-Xia Zhao1, Zeng-Gui Gao, Jing-Hua Zhuang
1Ministry of Agriculture Key Laboratory of Northern Crop Immunology, College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China. zhaobaixia1979@126.com
Abstract:
A total of 112 Fusarium isolates were obtained from 36 soil samples collected from the greenhouse melon fields of Liaoning Province, among which, 11 species were identified by traditional morphological classification and rDNA sequence analysis. Universally Primed PCR (UP-PCR) was conducted to analyze the 25 strains of test Fusarium isolates and 3 strains of positive control Fusarium isolates. The results indicated that a total of 73 bands appeared after amplification by using 6 primers, and 66 bands (90.4%) were polymorphic. The isolates were clustered into eight groups at the similarity of 0.736 by cluster analysis, among which, 14 isolates were clustered into one group. It was concluded that UP-PCR could present the genetic relationship and difference among Fusarium strains, being able to be used as an assistant method for Fusarium classification.
