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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

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[Lipid peroxidation and antioxidative protection in patients with coronary heart disease]

O L Belaia, N E Artamoshina, V I Kalmykova

    Klinicheskaia Meditsina
    |July 2, 2009
    PubMed

    Insights

    Patients with severe coronary heart disease (CHD) exhibit higher lipid peroxidation products (LPP) and lower antioxidant activity. These markers correlate with lipid composition and homocysteine in stable CHD.

    Area of Science:

    • Biochemistry
    • Cardiology
    • Clinical Chemistry

    Background:

    • Coronary heart disease (CHD) involves complex biochemical alterations.
    • Lipid peroxidation products (LPP) and oxidative stress are implicated in CHD pathogenesis.
    • Assessing antioxidant system activity is crucial for understanding disease severity.

    Purpose of the Study:

    • To investigate the relationship between lipid profiles, LPP, homocysteine, and antioxidant enzyme activity in CHD patients.
    • To differentiate biochemical markers between functional classes of angina (FC II and FC III).

    Main Methods:

    • Measurement of total cholesterol, HDL, LDL, and LPP (dienic conjugates, TBA-reactive compounds) in blood samples.
    • Quantification of plasma homocysteine and erythrocyte antioxidant enzyme activities (superoxide dismutase, glutathione peroxidase).
    • Assessment of plasma ceruloplasmin/transferrin antioxidant system.

    Main Results:

    • Patients with FC III angina showed significantly elevated LPP levels compared to FC II angina or healthy subjects.
    • Lower activity of tissue and plasma antioxidant systems was observed in FC III angina patients.
    • Stable CHD patients demonstrated correlations between lipid composition, LPP, homocysteine, and antioxidant enzyme activity.

    Conclusions:

    • Increased LPP and reduced antioxidant capacity are associated with more severe angina in CHD.
    • Biochemical markers including lipids, LPP, homocysteine, and antioxidant enzymes are interconnected in stable CHD.
    • These findings highlight the role of oxidative stress and lipid metabolism in CHD progression.

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