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Updated: Jun 22, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
Published on: February 24, 2026
High spatial resolution confocal microscope with independent excitation and detection scanning capabilities
S Marcet1, C Ouellet-Plamondon, S Francoeur
1Departement de Génie Physique, Ecole Polytechnique de Montréal, Montréal, Québec H3C 3A7, Canada.
Abstract:
We present the design of a confocal microscope adapted for optical spectroscopy and imaging at cryogenic temperatures. This system is based on the existing approach of partly inserting the optical components of the microscope inside a helium-bath cryostat. It provides a spatial resolution approaching the diffraction limit with a mechanical stability allowing uninterrupted integration times exceeding 10 h and allows keeping track of a single emitter for unlimited periods of time. Furthermore, our design allows scanning the excitation spot and detection area independently of the sample position. This feature provides the means to perform probeless transport experiments on one-dimensional nanostructures. The scanning capabilities of this microscope are fully detailed and characterized using the photoluminescence of single nitrogen dyads at 4.5 K.
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