Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 22, 2026

Quantification of Oculomotor Responses and Accommodation Through Instrumentation and Analysis Toolboxes
08:27

Quantification of Oculomotor Responses and Accommodation Through Instrumentation and Analysis Toolboxes

Published on: March 3, 2023

Halton sampling for autofocus.

T Pengo1, A Muñoz-Barrutia, C Ortiz-De-Solórzano

  • 1Cancer Imaging Laboratory, Center of Applied Medical Research, University of Navarra and Electrical, Electronic and Automation Engineering, School of Engineering, University of Navarra (TECNUN), Spain. tpengo@unav.es

Journal of Microscopy
|July 2, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automatic determination of the handedness of single-particle maps of macromolecules solved by CryoEM.

Journal of structural biology·2022
Same author

Image processing tools for the validation of CryoEM maps.

Faraday discussions·2022
Same author

Spheroscope: A custom-made miniaturized microscope for tracking tumour spheroids in microfluidic devices.

Scientific reports·2020
Same author

Computed Tomography-Based Biomarker for Longitudinal Assessment of Disease Burden in Pulmonary Tuberculosis.

Molecular imaging and biology·2018
Same author

Validation tool for traction force microscopy.

Computer methods in biomechanics and biomedical engineering·2014
Same author

Numerical estimation of 3D mechanical forces exerted by cells on non-linear materials.

Journal of biomechanics·2012
Same journal

Nanoprecipitate characterisation in NiTi and aluminium alloys: A TEM-based overview.

Journal of microscopy·2026
Same journal

BioImageIT: A novel python-based architecture for reproducible bio-image workflows.

Journal of microscopy·2026
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
See all related articles

A new Halton point set sampling strategy significantly improves autofocusing performance in automated microscopy. This method reduces computation time and bleaching while maintaining image accuracy for high-throughput screening.

Area of Science:

  • Microscopy
  • Image Analysis
  • Computational Imaging

Background:

  • Autofocusing is crucial for automated microscopy, ensuring image sharpness for accurate analysis.
  • Current autofocusing algorithms rely on contrast functions, optimization, and sampling strategies.
  • Sampling strategy, vital for high-throughput screening, balances sparse data acquisition with interpolation fidelity.

Purpose of the Study:

  • To introduce and evaluate a novel sampling strategy for autofocusing algorithms.
  • To compare the performance of the proposed Halton point set strategy against traditional methods like square grids and hexagonal lattices.

Main Methods:

  • The study proposes a new autofocus sampling strategy based on Halton point sets.
  • Performance was evaluated across nine focus surfaces under 24 Signal-to-Noise ratio (SNR) and sampling rate combinations.

More Related Videos

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

Related Experiment Videos

Last Updated: Jun 22, 2026

Quantification of Oculomotor Responses and Accommodation Through Instrumentation and Analysis Toolboxes
08:27

Quantification of Oculomotor Responses and Accommodation Through Instrumentation and Analysis Toolboxes

Published on: March 3, 2023

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

  • Comparison was made against classical square grid and hexagonal lattice sampling methods.

    • Main Results:

    • The Halton point set sampling strategy demonstrated superior performance in 88% of tested conditions.
    • This new strategy effectively reduces computation time and minimizes photobleaching.
    • It allows for faithful interpolation of the focus surface even with sparse sampling.

    Conclusions:

    • Halton sampling offers a more efficient and effective approach for autofocusing in automated microscopy.
    • The proposed strategy enhances image quality and reduces experimental overhead in high-content screening.
    • This advancement is particularly beneficial for reducing bleaching and computation time in demanding imaging applications.