Related Experiment Video
Updated: Jun 22, 2026

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders
Published on: April 3, 2021
[MyoD mRNA expression in skeletal muscle of patients with myotonic dystrophy]
Xiao-Ping Zhao1, Chuan-Qiang Pu, Feng Duan
1Department of Neurology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
Objective:
To explore the mRNA expression of MyoD gene in the skeletal muscles of myotonic dystrophy (MD) patients.
Methods:
Muscle biopsy specimens were obtained from the biceps muscles of arm of 4 MD patients and 4 healthy controls. Semi-quantitative reverse transcription polymerase chain reaction was performed to evaluate the mRNA expression of MyoD in the specimens.
Results:
The mRNA expression index of MyoD of the MD patients was (0.267 +/- 0.114), significantly lower than that of the healthy controls [(0.788 +/- 0.136), P = 0.001].
Conclusion:
Down-regulated mRNA expression of MyoD gene is involved in the mechanism of DM.
Related Concept Videos
Satellite Stem Cells and Muscular Dystrophy
Alterations in Muscle Tone lll
Formation of Muscle Fibers from Myoblasts
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
Myasthenia Gravis: Diagnostic Tests
The edrophonium test is a diagnostic tool for myasthenia gravis. It involves...
Disorders of the Skeletal Muscle
Musculoskeletal disorders
Musculoskeletal disorders involve injuries and conditions affecting the skeletal muscles and associated connective tissues. These disorders can arise from acute biomechanical stresses or chronic overuse and can occur across different age groups. Common injuries include sprains, fractures, and muscular strains, often resulting from...

