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Related Experiment Video

Updated: Jun 22, 2026

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
11:06

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro

Published on: January 31, 2022

A microfluidic 3D hepatocyte chip for drug toxicity testing.

Yi-Chin Toh1, Teck Chuan Lim, Dean Tai

  • 1Institute of Bioengineering and Nanotechnology, A*STAR, The Nanos, Singapore, 138669, Singapore.

Lab on a Chip
|July 2, 2009
PubMed
Summary

A novel 3D HepaTox Chip uses microfluidic technology for in vitro drug toxicity testing. This advanced platform accurately predicts in vivo drug hepatotoxicity by mimicking liver cell functions.

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Area of Science:

  • Biotechnology
  • Drug Discovery
  • Toxicology

Background:

  • Hepatotoxicity is a major concern in drug development.
  • Existing in vitro models often fail to accurately predict in vivo drug responses.
  • Need for advanced platforms to assess drug-induced liver injury.

Purpose of the Study:

  • To develop and validate a microfluidic 3D hepatocyte chip (3D HepaTox Chip) for in vitro drug toxicity testing.
  • To accurately predict in vivo drug hepatotoxicity using an engineered 3D microenvironment.
  • To establish a dose-dependent drug response assay for predicting liver toxicity.

Main Methods:

  • Engineered a 3D microenvironment within multiplexed microfluidic channels.
  • Utilized functional primary hepatocytes to maintain synthetic and metabolic functions.

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Last Updated: Jun 22, 2026

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro
11:06

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro

Published on: January 31, 2022

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture
10:05

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

Published on: April 28, 2015

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
12:39

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

Published on: May 21, 2008

  • Incorporated a concentration gradient generator for dose-dependent drug administration and response analysis.

    • Main Results:

    • The 3D HepaTox Chip successfully maintained hepatocyte functions.
    • In vitro dose-dependent drug responses were accurately measured.
    • IC(50) values from the chip correlated well with reported in vivo LD(50) values for 5 model drugs.

    Conclusions:

    • The 3D HepaTox Chip is a promising tool for in vitro drug hepatotoxicity testing.
    • This platform can improve the prediction of in vivo drug safety.
    • Future integration with sensors and actuators will enhance its capabilities as a next-generation drug testing platform.