Related Experiment Video
Updated: Jun 22, 2026
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Recent advances in chemical proteomics: exploring the post-translational proteome
1Department of Chemistry, Imperial College London, Exhibition Road, London, SW72AZ, UK, e.tate@imperial.ac.uk.
Journal of Chemical Biology
|July 2, 2009
Summary
Chemical proteomics offers new ways to study protein modifications. This field uses chemical tools to understand the function of post-translational modifications (PTMs) in biology.
Area of Science:
- Biochemistry
- Molecular Biology
- Chemical Biology
Background:
- Protein identification and quantification from complex biological samples are crucial in post-genomic research.
- Analyzing post-translational modifications (PTMs) in proteomics remains a significant challenge despite advances in high-throughput techniques.
Purpose of the Study:
- To introduce the emerging field of chemical proteomics.
- To review recent technological advancements in chemical proteomics.
- To highlight new insights into the functional biology of PTMs provided by chemical proteomics.
Main Methods:
- Review of recent literature on chemical proteomic technologies.
- Focus on methods enabling the study of post-translational modifications.
Main Results:
- Chemical proteomics is an emerging field with significant potential.
- Recent advances in chemical proteomic technology provide novel tools for PTM analysis.
- These technologies offer striking new insights into the functional biology of PTMs.
Conclusions:
- Chemical proteomics is a rapidly developing area.
- Technological advances in chemical proteomics are crucial for understanding PTMs.
- This approach provides valuable insights into the functional roles of post-translational modifications.
Related Concept Videos
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
01:33Peptide Identification Using Tandem Mass Spectrometry
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...