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Published on: February 11, 2019
Mechanisms of protein stabilization in the solid state
Liuquan Lucy Chang1, Michael J Pikal
1Wyeth Vaccine Research and Development, Pearl River, NY 10965, USA. changl5@wyeth.com
Journal of Pharmaceutical Sciences
|July 2, 2009
Summary
Many proteins degrade in the solid state. This review covers protein degradation pathways and stabilization strategies, crucial for pharmaceutical formulation development.
Area of Science:
- Biochemistry and Pharmaceutical Sciences
- Protein chemistry and solid-state stability
Background:
- Proteins exhibit marginal stability in solution, making the solid state preferable for storage.
- Solid-state proteins are susceptible to chemical and physical degradation during processing and use.
Purpose of the Study:
- To review major protein degradation pathways in the solid state.
- To summarize effective protein stabilization strategies.
- To elucidate the mechanisms underlying protein stabilization in solid formulations.
Main Methods:
- Literature review of protein degradation mechanisms.
- Analysis of stabilization strategies for solid-state proteins.
- Discussion of formulation development principles.
Main Results:
- Identified key chemical and physical degradation routes for solid-state proteins.
- Outlined various approaches to enhance protein stability in solid forms.
- Highlighted the importance of understanding stabilization mechanisms.
Conclusions:
- Understanding protein degradation and stabilization mechanisms is vital for successful pharmaceutical formulation.
- Effective stabilization strategies are essential for maintaining protein integrity in the solid state.
- This knowledge underpins the development of stable and effective protein-based therapeutics.
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