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Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
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Mobile phase containing arginine provides more reliable SEC condition for aggregation analysis.

Ryosuke Yumioka1, Haruna Sato, Hiroshi Tomizawa

  • 1AminoScience Laboratories, Ajinomoto Co., Inc., Kawasaki-ku, Kawasaki, Japan.

Journal of Pharmaceutical Sciences
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PubMed
Summary

Arginine addition to size exclusion chromatography (SEC) columns reduces protein aggregate loss. This ensures accurate aggregate content measurement in pharmaceutical protein quality control, even with new columns.

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Area of Science:

  • Protein aggregation analysis
  • Chromatographic methods
  • Pharmaceutical quality control

Background:

  • Protein aggregate loss during size exclusion chromatography (SEC) leads to underestimation of aggregate content in pharmaceutical protein quality control.
  • New SEC columns exhibit increased protein binding, exacerbating aggregate loss.
  • Suppression of protein adsorption is crucial for reliable aggregate quantification.

Purpose of the Study:

  • To investigate the effect of arginine on protein binding to SEC columns.
  • To determine if arginine can mitigate protein aggregate loss during SEC.
  • To establish a reliable method for pharmaceutical protein aggregate assessment.

Main Methods:

  • Size Exclusion Chromatography (SEC) was employed to analyze protein samples.
  • The impact of arginine on protein-column interactions was evaluated.
  • Sodium chloride (NaCl) was used as a control to assess ionic strength effects.

Main Results:

  • Arginine significantly reduced the loss of protein aggregates during SEC.
  • The presence of arginine enabled accurate aggregate content estimation, even with new columns and low protein loads.
  • Increased ionic strength from NaCl led to greater protein loss, unlike arginine.

Conclusions:

  • Arginine effectively suppresses protein adsorption to SEC columns, preventing aggregate loss.
  • Arginine supplementation provides a reliable method for quantifying pharmaceutical protein aggregates.
  • This finding is critical for improving the quality control of protein-based therapeutics.