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Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
Published on: November 30, 2015
Rapid ABO genotyping using whole blood without DNA purification
Sung Ho Lee1, Geon Park, Young Geun Yang
1R&D Center, Lumieye Genetics Co Ltd, Seongbuk-Gu, Seoul, Korea.
The Korean Journal of Laboratory Medicine
|July 3, 2009
Summary
A new multiplex allele-specific PCR (ASPCR) assay enables ABO genotyping directly from whole blood samples. This method accurately determines blood group genotypes, offering a valuable alternative to traditional serological typing.
Area of Science:
- Genetics
- Molecular Biology
- Forensic Science
Background:
- ABO genotyping is crucial for resolving discrepancies in blood typing and for forensic applications like personal identification and paternity testing.
- Traditional methods can be time-consuming and may require purified DNA.
Purpose of the Study:
- To develop and evaluate a novel multiplex allele-specific PCR (ASPCR) assay for ABO genotyping.
- To assess the assay's performance using whole blood samples without prior DNA purification.
Main Methods:
- A four-reaction multiplex ASPCR assay was designed to detect specific nucleotide differences among six ABO alleles (A101, A102, B101, O01, O02, cis-AB01).
- ABO genotypes of 127 randomly selected samples were determined using the multiplex ASPCR method.
- The assay was performed directly on whole blood samples.
Main Results:
- The multiplex ASPCR assay successfully determined the ABO genotypes for all 127 samples, with detailed genotype distributions provided.
- Calculated phenotypes from the genotypes matched serological findings.
- Comparison with DNA sequencing confirmed the accuracy of the multiplex ASPCR assay, showing no discrepancies.
Conclusions:
- The developed multiplex ASPCR assay is a convenient and effective method for ABO genotyping.
- This assay, usable with whole blood, supplements routine serological ABO typing.
- The method may also find utility in other genotyping applications.
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