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Helicobacter pylori increases expression of proapoptotic markers Fas and FasL on CD4 lymphocytes in children

Aldona Kotłowska-Kmieć1, Alicja Bakowska, Adam Szarszewski

  • 1Children's Hospital, Polanki 119, Gdańsk, Poland. akmiec@amg.gda.pl

Acta Biochimica Polonica
|July 3, 2009
PubMed

Insights

Helicobacter pylori infection in children involves specific B and T helper lymphocytes and Fas receptor expression in gastric mucosa. Eradicating H. pylori reduces these inflammatory markers, suggesting a role in gastritis pathogenesis.

Area of Science:

  • Pediatric Gastroenterology
  • Immunology
  • Microbiology

Background:

  • The precise mechanisms of Helicobacter pylori's impact on gastric mucosa and its contribution to gastritis pathogenesis remain unclear.
  • Understanding the role of lymphocytes and apoptosis markers in pediatric H. pylori-associated gastritis is crucial.

Purpose of the Study:

  • To identify prevalent lymphocyte subpopulations in the gastric mucosa of children with gastritis.
  • To assess the expression of Fas and Fas ligand (FasL) in gastric mucosa lymphocytes before and after H. pylori eradication.
  • To explore the correlation between lymphocyte populations, Fas/FasL expression, and H. pylori infection in children.

Main Methods:

  • Immunohistochemistry was used to analyze gastric mucosa tissue samples from 49 pediatric patients (ages 6-17).
  • Lymphocyte subsets (CD3, CD4, CD8, CD20), cytokines (IL-4, IL-6, IFNgamma), and Fas/FasL proteins were quantified.
  • Analysis was performed before and after H. pylori eradication therapy.

Main Results:

  • B and T helper lymphocytes (CD4+) were significantly involved in inflammatory infiltration of the gastric mucosa during H. pylori infection.
  • Enhanced expression of the Fas receptor was observed on lymphocytes prior to H. pylori eradication.
  • Successful eradication led to a decrease in both lymphocyte subpopulations and Fas receptor expression.

Conclusions:

  • B and T helper lymphocytes play a key role in pediatric H. pylori-induced gastritis.
  • Fas receptor expression on lymphocytes is elevated during active infection and decreases post-eradication.
  • A correlation between CD4+ lymphocyte and Fas receptor expression suggests a mechanism for apoptosis induction in infected children.

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