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Updated: Jun 22, 2026
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
Published on: May 16, 2022
Rapid Raman imaging of stable, functionalized nanoshells in mammalian cell cultures
Yiming Huang1, Vimal P Swarup, Sandra Whaley Bishnoi
1BCPS Department, Illinois Institute of Technology, 3101 South Dearborn, Chicago, IL, USA.
Abstract:
Two Raman-active poly(ethylene glycol) (PEG) molecules, one linear (MW 5000) and the other branched (MW 2420), are synthesized to stabilize gold-silica nanoshells in cell culture media and track nanoparticles in mammalian cell cultures. The linear PEG provides greater nanoshell stability in saline solution compared to commercially available PEG-thiol or the branched PEG. Surface enhanced Raman scattering rapidly tracks the probes and provides semiquantitative information regarding particle localization within mouse macrophage (RAW 264.7) and human breast cancer (MCF 7) cell cultures.
