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Evaluation of total oxidative and antioxidative status in pediatric patients undergoing laparoscopic surgery
Zeynep Baysal1, Turhan Togrul, Nurten Aksoy
1Department of Anesthesiology and Reanimation, Faculty of Medicine, Harran University, 63100 Sanliurfa, Turkey. zbaysal2003@yahoo.com
Insights
Laparoscopic surgery in children increases oxidative stress due to ischemia-reperfusion, depleting plasma antioxidants. This study measured oxidative-antioxidative status in pediatric patients undergoing these procedures.
Area of Science:
- Pediatric surgery
- Oxidative stress research
- Biochemistry
Background:
- Reactive oxygen species (ROS) contribute to oxidative stress in various diseases.
- Pneumoperitoneum during laparoscopy can cause splanchnic ischemia-reperfusion, a known inducer of oxidative stress.
Purpose of the Study:
- To investigate the oxidative-antioxidative status in pediatric patients undergoing laparoscopic surgery.
- To assess the impact of laparoscopic procedures on oxidative stress markers.
Main Methods:
- Blood samples were collected from pediatric patients after anesthesia induction and at the end of laparoscopic surgery.
- Plasma levels of total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were determined.
Main Results:
- Plasma TOS and OSI levels significantly increased by the end of surgery compared to post-anesthesia induction (P < .05 and P < .01).
- Plasma TAS levels significantly decreased by the end of surgery (P < .05).
Conclusions:
- Laparoscopic procedures in pediatric patients generate ROS, likely from ischemia-reperfusion induced by pneumoperitoneum.
- The observed changes indicate increased oxidative stress and consumption of plasma antioxidants during laparoscopic surgery.
Objective:
Reactive oxygen species (ROS) induced by several diseases can trigger oxidative stress. During laparoscopy, increased intraabdominal pressure caused by pneumoperitoneum may lead to splanchnic ischemia followed by reperfusion because of deflation. Because ischemia reperfusion creates oxidative stress, in this study, we aimed to investigate the oxidative-antioxidative status of the pediatric patients with laparoscopic surgery.
Methods:
The children underwent laparoscopic procedures under general anesthesia, and they were mechanically ventilated. Blood samples were obtained after induction of anesthesia, at the end of the surgery, and were centrifuged at 3000 revolutions per minute for 10 minutes to separate plasma. The plasma total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were determined.
Results:
Plasma TOS and OSI levels were significantly higher at the end of the surgery than after induction of anesthesia (P < .05 and P < .01, respectively). On the other hand, plasma TAS levels were lower in the end of the surgery than after induction of anesthesia (P < .05).
Conclusions:
These results suggest that ROS are generated during the laparoscopic procedure, possibly as a result of an ischemia-reperfusion phenomenon induced by the inflation and deflation of the pneumoperitoneum, which causes oxidative stress and consume plasma antioxidants.
