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Movement Retraining using Real-time Feedback of Performance
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Laboratory issues in the implementation of the marker method.

David A Cowan1, Christiaan Bartlett

  • 1Drug Control Centre, Department of Forensic Science and Drug Monitoring, King's College London, London SE1 9NH, United Kingdom. David.cowan@kcl.ac.uk

Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society
|July 4, 2009
PubMed
Summary
This summary is machine-generated.

Insulin-like growth factor I (IGF-I) and its precursor, P-III-P, are key biomarkers for detecting growth hormone administration. Current assays for P-III-P are limited, impacting doping control effectiveness.

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Area of Science:

  • Biochemistry
  • Sports Science
  • Analytical Chemistry

Background:

  • Growth hormone (GH) and Insulin-like Growth Factor I (IGF-I) administration are prohibited in sports.
  • Accurate detection of GH/IGF-I abuse relies on reliable biomarkers.
  • IGF-I and its precursor, the pro-peptide of IGF-I (P-III-P), are established biomarkers.

Purpose of the Study:

  • To review available assays for IGF-I and P-III-P.
  • To discuss the suitability of these assays for doping control.
  • To explore future analytical possibilities.

Main Methods:

  • Review of commercially available immunoassays for IGF-I and P-III-P.
  • Discussion of assay principles and performance characteristics.
  • Consideration of mass spectrometry for future quantification.

Main Results:

  • Multiple assays exist for IGF-I, but only two are available for P-III-P.
  • Assay selection for doping control requires careful consideration of sensitivity, specificity, and matrix effects.
  • Mass spectrometry offers potential for improved accuracy and multiplexing.

Conclusions:

  • The limited availability of P-III-P assays poses a challenge for doping control.
  • Further development of robust P-III-P assays is needed.
  • Mass spectrometry represents a promising future direction for biomarker quantification in anti-doping.