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Related Experiment Video

Updated: Jun 21, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

Prenatal Group B Streptococcus test using real-time polymerase chain reaction.

Chi-Feng Wei1, Bo-Ching She, Hung-Shuo Liang

  • 1Department of Obstetrics and Gynecology, Cathay General Hospital, Si-Jhih, Taiwan.

Taiwanese Journal of Obstetrics & Gynecology
|July 4, 2009
PubMed
Summary

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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A small percentage of pregnant women screened negative for Group B Streptococcus (GBS) at 35 weeks later tested positive. Repeat GBS testing after 39 weeks is recommended to ensure accurate screening.

Area of Science:

  • Obstetrics and Gynecology
  • Microbiology
  • Infectious Diseases

Background:

  • Group B Streptococcus (GBS) screening is crucial for preventing neonatal infections.
  • Standard GBS culture screening occurs between 35-37 weeks of gestation.
  • False-negative GBS screening results can lead to missed opportunities for treatment.

Purpose of the Study:

  • To determine the rate of GBS positivity in pregnant women with initially negative culture results after 39 weeks' gestation.
  • To assess the reliability of late-term GBS screening.

Main Methods:

  • Prospective study of 150 pregnant women with negative GBS cultures at 35 weeks' gestation.
  • Repeat GBS testing using culture and real-time polymerase chain reaction (RT-PCR) after 39 weeks' gestation.

Related Experiment Videos

Last Updated: Jun 21, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

  • Analysis of GBS status in late pregnancy.

    • Main Results:

    • Two percent of pregnant women with negative GBS cultures at 35 weeks tested positive after 39 weeks' gestation.
    • This indicates a potential for false-negative results with standard screening timing.
    • RT-PCR demonstrated a rapid method for detecting GBS.

    Conclusions:

    • A GBS test 4 weeks after an initial negative screen (at 35-37 weeks) is necessary.
    • Real-time PCR is a viable, rapid alternative for detecting GBS colonization near labor.
    • Optimizing GBS screening protocols can improve neonatal outcomes.