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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Related Experiment Video

Updated: Jun 21, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
09:42

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Production of polyclonal antisera.

Helen M Cooper1, Yvonne Paterson

  • 1Queensland Brain Institute and University of Queensland, Brisbane, Australia.

Current Protocols in Neuroscience
|July 4, 2009
PubMed
Summary
This summary is machine-generated.

Generating specific polyclonal antibodies is crucial for biological research techniques. This guide offers protocols for producing these essential antibodies in common laboratory animals.

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Last Updated: Jun 21, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
09:42

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Generation of Monoclonal Antibodies Against Natural Products
12:15

Generation of Monoclonal Antibodies Against Natural Products

Published on: April 6, 2019

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
13:14

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Area of Science:

  • Biochemistry
  • Immunology
  • Molecular Biology

Background:

  • Specific antibodies are essential for numerous biological and biochemical techniques.
  • Techniques like immunohistochemistry, ELISAs, immunoprecipitation, and immunoblotting rely heavily on antibody availability.
  • Antibody-based proteomics necessitates the timely production of specific antibodies.

Purpose of the Study:

  • To provide protocols for generating polyclonal antisera.
  • To detail methods for producing specific antibodies against protein antigens.
  • To outline antibody production in various animal models.

Main Methods:

  • Immunization of animals (rabbits, rats, mice, hamsters) with purified protein or peptide antigens.
  • Collection and processing of antisera from immunized animals.
  • Validation of antibody specificity and quality.

Main Results:

  • Established protocols for polyclonal antibody production.
  • Demonstrated successful generation of specific antibodies in multiple animal models.
  • Provided a resource for researchers needing reliable antibody production methods.

Conclusions:

  • Polyclonal antibodies are valuable tools for basic and applied research.
  • The provided protocols facilitate the generation of specific antibodies for diverse applications.
  • Efficient antibody production is critical for advancing biological and proteomic studies.