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Updated: Jun 21, 2026
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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
Published on: August 5, 2020
Laser microdissection-mediated isolation and in vitro transcriptional amplification of plant RNA
Michael J Scanlon1, Kazuhiro Ohtsu, Marja C P Timmermans
1Cornell University, Ithaca, New York, USA.
Current Protocols in Molecular Biology
|July 4, 2009
Abstract:
Protocols for laser microdissection and linear amplification of RNA from fixed, sectioned plant tissues are described. When combined with quantitative RT-PCR, microarray analysis, or RNA-sequencing, these procedures enable quantitative analyses of transcript accumulation from microscopic quantities of specific plant organs, tissues, or single cells.
