Related Experiment Video
Updated: Jun 21, 2026
In-Vivo Calcium Imaging of Sensory Neurons in the Rat Trigeminal Ganglion
Published on: February 9, 2024
[Nav1.8 and Nav1.9 mRNA expression in rat trigeminal ganglion at different interval after molar extraction]
Lei Zhang1, Hong-Chen Liu, Dong-Sheng Wang
1Stomatological Center, General Hospital of PLA, Beijing 100853, China.
Objective:
To observe the expression and function of extraction.
Methods:
Real-time reverse transcription PCR paralleled with vitro-established cRNA standard curves was applied to measure the expression of Nav1.8, Nav1.9 at 30 min, 2 h, 1 d, 3 d and 6 d respectively after extraction of rat right mandibular molars. The right mandibular molars were used as control.
Results:
Both Nav1.8 and Nav1.9 mRNA in right trigeminal ganglion showed little change after 30 min, and increased slowly after 2 h. Nav1.8, Nav1.9 mRNA expressions increased by 27% and 24.5% respectively compared to the left trigeminal ganglion after 3 d, reaching the highest level (P < 0.05), and then the expressions began decreasing from 6 d.
Conclusions:
The pain caused by molar extraction is related to the up-regulation of expressions of sodium channels protein Nav1.8 and Nav1.9 mRNA, indicating the participation of sodium channels in regulations of peripheral tissue pain after molar extraction.
