[A differential expression proteomic study of human periodontal ligament cell during osteogenic differentiation]
Li-ping Wu1, Xi Wei, Jun-qi Ling
1Department of Cariology and Endodontology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
Objective:
To obtain regulating proteins during human periodontal ligament cell (hPDLC) osteogenic differentiation and investigate its underlying molecular mechanism.
Methods:
Two-dimension difference in gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser adsorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) analysis was used to identify regulating proteins during hPDLC osteogenic differentiation.
Results:
A differently expressed protein profile was obtained 7 days after osteogenic induction, and 61 protein spots with significant differences, 29 protein spots were identified by MALDI-TOF-MS, including cytoskeleton, cell membrane-bounding, nuclear regulation, matrix synthesis, and metabolic enzymes and signal transduction and other proteins. A functional class of cytoskeleton proteins showed co-regulation, these proteins were intimately involved in the reorganization of the cytoskeleton, cytokinesis and migration during PDLC differentiation.
Conclusions:
A database of differently displayed proteins during hPDLC osteogenic differentiation was established, which may be helpful to understand and further study molecular mechanism of PDLC osteogenic differentiation.