Related Experiment Video
Updated: Jun 21, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Published on: December 20, 2014
Regulation of centrosome separation in yeast and vertebrates: common threads
Hong Hwa Lim1, Tao Zhang, Uttam Surana
1Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Proteos, 138673 Singapore.
Abstract:
The assembly of a bipolar spindle is crucial for symmetric partitioning of duplicated chromosomes during cell division. Centrosomes (spindle pole body [SPB] in yeast) constitute the two poles of this bipolar structure and serve as microtubule nucleation centers. A eukaryotic cell enters the division cycle with one centrosome and duplicates it before spindle formation. A proteinaceous link keeps duplicated centrosomes together until it is severed at onset of mitosis, enabling centrosomes to migrate away from each other and assemble a characteristic mitotic spindle. Hence, centrosome separation is crucial in assembly of a bipolar spindle. Whereas centrosome (or SPB) duplication has been characterized in some detail, the separation process is less well understood. Here, we review recent studies that uncover new players and provide a greater understanding of the regulation of centrosome (or SPB) separation.
Related Concept Videos
Centrioles and Centrosomes
Near the end of the prophase, also called late prophase or "prometaphase,"...
Centrosome Duplication
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Separation of Sister Chromatids
At the onset of anaphase, separase, a proteolytic enzyme, is...
Separation of Sister Chromatids
At the onset of anaphase, separase, a proteolytic enzyme, is...
Meiosis II
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...

