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Alkaline pH enhances farnesol production by Saccharomyces cerevisiae
Masayoshi Muramatsu1, Chikara Ohto, Shusei Obata
1Bio Research Lab., Toyota Motor Corporation, Toyota 471-8572, Japan.
Abstract:
External environments affect prenyl alcohol production by squalene synthetase-deficient mutant Saccharomyces cerevisiae ATCC 64031. Cultivation of the yeast in medium with an initial pH ranging from 7.0 to 8.0 increased the amount of secreted farnesol (FOH). In contrast, acidic medium with a pH below 4.0 increased the intracellular FOH and its isomer nerolidol. These effects of alkaline pH were also observed on constant pH cultivation in a jar fermenter. On cultivation for 133 h, the FOH production reached 102.8 mg/l.
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