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Related Concept Videos

Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Introduction to Enzyme Kinetics01:19

Introduction to Enzyme Kinetics

Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...
Production of Organic Acids01:25

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

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Related Experiment Video

Updated: Jun 21, 2026

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
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High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Published on: September 15, 2015

Modeling cellulase kinetics on lignocellulosic substrates.

Prabuddha Bansal1, Mélanie Hall1, Matthew J Realff1

  • 1School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, N.W., Atlanta, GA 30332-0100, USA.

Biotechnology Advances
|July 7, 2009
PubMed
Summary

Cellulase enzyme hydrolysis of cellulose for biofuel production faces rate slowdowns. This review analyzes kinetic models to improve enzyme and biomass conversion efficiency for economic viability.

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High-throughput Saccharification Assay for Lignocellulosic Materials

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Published on: July 3, 2011

Area of Science:

  • Biomass Conversion
  • Enzymatic Hydrolysis
  • Biofuel Production

Background:

  • Cellulase enzymatic hydrolysis of cellulose is key for lignocellulosic biomass to biofuel conversion.
  • The process is limited by a significant rate slowdown at high conversion levels, hindering economic viability.

Purpose of the Study:

  • To review and analyze kinetic models for cellulose enzymatic hydrolysis.
  • To classify and tabulate existing models and their underlying assumptions.

Main Methods:

  • Literature review of kinetic models for cellulose hydrolysis.
  • Classification and tabulation of models based on assumptions and rate-limiting factors.

Main Results:

  • Identified various factors influencing enzymatic rates and activities through existing models.
  • Highlighted the need for further experimental validation of model hypotheses.

Conclusions:

  • Kinetic models have elucidated factors affecting cellulose hydrolysis but require more experimental data.
  • Future models should incorporate additional substrate and enzyme properties for improved accuracy.