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Related Experiment Video

Updated: Jun 21, 2026

Protocols for Robust Herbicide Resistance Testing in Different Weed Species
10:52

Protocols for Robust Herbicide Resistance Testing in Different Weed Species

Published on: July 2, 2015

A Rapid and Simple Bioassay Method for Herbicide Detection.

Xiu-Qing Li1, Alan Ng, Russell King

  • 1Agriculture and Agri-Food Canada, 850 Lincoln Road, P.O. Box 20280, Fredericton, NB, E3B 4Z7, Canada.

Biomarker Insights
|July 7, 2009
PubMed
Summary

A new algal lawn-agar assay using Chlamydomonas reinhardtii enables semi-quantitative detection of herbicide activity. This simple method can screen for herbicide contamination in water samples quickly and affordably.

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Algology

Background:

  • * Chlamydomonas reinhardtii is a unicellular green alga utilized in bioassays for toxic compounds.
  • * Traditional bioassays often rely on liquid culture systems, which can be complex.
  • * There is a need for simpler, semi-quantitative methods for detecting specific contaminants like herbicides.

Purpose of the Study:

  • * To develop and validate an algal lawn-agar system for the semi-quantitative bioassay of herbicidal activities.
  • * To assess the efficacy of this system in detecting various herbicides.
  • * To establish a rapid and cost-effective screening tool for herbicide contamination.

Main Methods:

  • * An algal lawn-agar system was established using Chlamydomonas reinhardtii.

More Related Videos

Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay
10:49

Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay

Published on: September 7, 2015

Related Experiment Videos

Last Updated: Jun 21, 2026

Protocols for Robust Herbicide Resistance Testing in Different Weed Species
10:52

Protocols for Robust Herbicide Resistance Testing in Different Weed Species

Published on: July 2, 2015

Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay
10:49

Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay

Published on: September 7, 2015

  • * Sixteen herbicides from 11 categories were applied to paper disks and placed on algal lawns in Petri dishes.
  • * Clearing zones around paper disks were observed and measured 2-3 days post-application.

    • Main Results:

    • * The algal lawn-agar system successfully indicated the presence of herbicidal activity through observable clearing zones.
    • * The size of the clearing zones varied based on herbicide concentration, mode of action, and chemical properties.
    • * The system demonstrated semi-quantitative potential in differentiating herbicidal effects.

    Conclusions:

    • * A novel, simple paper-disk-algal assay was developed for semi-quantitative herbicide detection.
    • * This method offers a quick, inexpensive screening tool for assessing herbicide contamination in environmental samples, such as water.
    • * The Chlamydomonas reinhardtii lawn-agar system provides a practical approach for preliminary environmental monitoring.