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Published on: March 30, 2018
Mutational analysis of Arabidopsis PP2CA2 involved in abscisic acid signal transduction
Xin-Hong Guo1, Ke-Qin Deng, Jie Wang
1Institute of Life Science and Biotechnology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, 410082, Changsha, People's Republic of China. xinhong@hnu.cn
Abstract:
The homozygous T-DNA mutant of the PP2CA2 gene in Arabidopsis thaliana was identified at DNA and RNA levels. The semi-quantitative RT-PCR analysis showed expression of PP2CA2 was induced by NaCl and ABA. When grown in presence of increasing concentration of exogenous ABA the pp2ca2 mutant showed a significant loss of ABA sensitivity in terms of seed germination, efficiency of post-germination growth and root growth. In presence of all ABA and NaCl concentrations tested the germination percentage of wild-type seeds was lower than that of mutant ppca2 seeds. Furthermore, in the presence of exogenous ABA, the pp2ca2 seeds showed higher germination percentages than wild-type at different stages of development and the pp2ca2 seedlings showed a reduced inhibition of root growth compared with wild-type plants. The above results indicated that the pp2ca2 was an ABA-hyposensitive mutant.

