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Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
Genetic diversity of grapevine accessions from Iran, Russia and USA using microsatellite markers
A Ramezani1, R Haddad, M Dorostkar
1Department of Agricultural Biotechnology, Imam Khomeini International University, Gazvin, Iran.
Abstract:
To discover marker information content and differentiation among grapevine accessions from Iran, USA and Russia, nine microsatellite markers were used. A total of 75 alleles were detected, giving a mean of 8.3 alleles per 9 loci. The total number of alleles per locus varied between 6 to 11 and the polymorphism information content ranged from 0.65 to 0.88, indicating that these loci were highly informative. A positive correlation (r = 0.870) was observed between the number of alleles and the level of polymorphism. Two SSRs loci including SSrVrZAG47 and VVMD27 were found to be probably synonymous. Gene diversities were high in all populations with values ranging from 0.709 to 0.784. In all populations, the mean number (averaged over loci) of heterozygous individuals was higher than expected. PCO analysis could not be so clearly differentiated accessions from Iran and Russia. The pattern of clustering of the Vitis vinifera populations was according to their geographic distribution. It is suggested that accessions could possibly be assigned to their regions of origin according to their genotypes.
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