Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sex-specific in utero reprogramming of lung immunity.

Mucosal immunology·2026

Same author

Metastasis suppressing properties of the cell-surface anchored serine protease prostasin: new functional and mechanistic insights from breast cancer.

Oncogenesis·2026

Same author

Integrative Proteomic and Bioenergetic Profiling Reveals Diet- and Strain-Specific Mitochondrial Dysfunction in Cohen Diabetic Rat Hearts.

Antioxidants (Basel, Switzerland)·2026

Same author

Global drivers of variation in blood mercury of seabirds revealed by a meta-analysis.

The Science of the total environment·2026

Same author

Genome assembly of the Antipodean and Gibson's albatrosses (Diomedea antipodensis antipodensis and D. a. gibsoni).

The Journal of heredity·2025

Same author

O-GlcNAc modification differentially regulates microtubule binding and pathological conformations of tau isoforms in vitro.

The Journal of biological chemistry·2025

Same journal

Inflammatory Preconditioning Reprograms Mesenchymal Stromal Cell-Derived Extracellular Vesicles into Distinct Functional States for Immunomodulation and Wound Healing.

Stem cells and development·2026

Same journal

Cancer Stem Cells as Dynamic Attractor States: Systems Biology of Tumor Plasticity and Therapy Resistance.

Stem cells and development·2026

Same journal

Evaluation of Puromycin-Induced Podocyte Injury and Protective Effects of Voclosporin Using Induced Pluripotent Stem Cells from a Patient with Nephrotic Syndrome Harboring an <i>INF2</i> Variant.

Stem cells and development·2026

Same journal

Simplified Derivation of Stable Early Passage Murine Embryonic and Parthenogenetic Stem Cells.

Stem cells and development·2026

Same journal

OCT3/4-Enhanced Dental Mesenchymal Stem Cells Acquire Hematopoietic Stem Cell-Like Properties and Support Hematopoietic Recovery in a Myelosuppressed Mouse Model.

Stem cells and development·2026

Same journal

Hematopoietic Stem Cell-Based Cell and Gene Therapy Beyond Monogenic Diseases.

Stem cells and development·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

Intestinal epithelial cells in vitro.

Dharam P Chopra¹, Alan A Dombkowski, Paul M Stemmer

¹Institute of Environmental Health Sciences, Wayne State University, Detroit, MI 48201, USA.

Stem Cells and Development

|July 8, 2009

Summary

This summary is machine-generated.

Developing long-term intestinal epithelial stem cell cultures is crucial for regenerative medicine and understanding digestive disorders. This review summarizes current in vitro propagation techniques and stem cell markers.

More Related Videos

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Published on: July 29, 2021

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

Published on: February 6, 2021

Related Experiment Videos

Last Updated: Jun 21, 2026

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Published on: July 29, 2021

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

Published on: February 6, 2021

Area of Science:

Gastroenterology
Stem Cell Biology
Regenerative Medicine

Background:

Advances in stem cell biology highlight the need for normal intestinal epithelial cell lines.
Current short-term models hinder research into stem/progenitor cell differentiation and digestive disorders.
A lack of long-term intestinal epithelial and stem cell cultures limits therapeutic development.

Purpose of the Study:

To review current technologies for in vitro propagation of intestinal epithelial cells.
To discuss markers for identifying intestinal stem cells.
To summarize gene and protein expression profiles during intestinal cell differentiation.

Main Methods:

Literature review of existing in vitro culture models for intestinal epithelial cells.
Analysis of identified intestinal stem cell markers.
Compilation of data on gene and protein expression in intestinal differentiation.

Main Results:

Several short-term culture models exist, but long-term propagation remains challenging.
Key markers for intestinal stem cells have been identified.
Gene and protein expression profiles are crucial for understanding differentiation pathways.

Conclusions:

There is a critical need for improved in vitro models of intestinal epithelial stem cells.
Further research into long-term culture and differentiation is essential for regenerative medicine.
Understanding stem cell markers and differentiation profiles will advance treatments for digestive diseases.