Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deletion of Snap25 disrupts glial remodeling in aging mouse brain.

iScience·2026
Same author

Presynaptic SNAP25 supports maturation of hippocampal mossy fiber-CA3 synapses.

iScience·2026
Same author

The MacBrain Resource Center (MBRC) rhesus macaque postnatal brain histology datasets: Enabling new discoveries through NHP tissue and digital data Repositories.

Journal of anatomy·2026
Same author

Anti-fibrotic effect of <i>Spirulina maxima</i>-derived extracellular vesicles: possible role of PARK7 and HSP70 chaperones.

Frontiers in bioengineering and biotechnology·2026
Same author

Lamination of primary visual cortex in the macaque: Layer 5 subdivisions.

Journal of anatomy·2026
Same author

The layer 6b theory of attention.

Neuron·2026

Related Experiment Video

Updated: Jun 21, 2026

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
10:06

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Published on: November 13, 2011

High quality RNA from multiple brain regions simultaneously acquired by laser capture microdissection.

Wei-Zhi Wang1, Franziska M Oeschger, Sheena Lee

  • 1Department of Physiology, Anatomy and Genetics, University of Oxford, Le Gros Clark Building, Oxford, UK. wei-zhi.wang@dpag.ox.ac.uk

BMC Molecular Biology
|July 8, 2009
PubMed
Summary

This study presents an optimized laser capture microdissection protocol for isolating high-quality RNA from small cell samples. The method ensures RNA integrity for gene expression studies in discrete cell populations.

More Related Videos

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
07:01

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

Published on: April 11, 2015

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
13:32

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Published on: April 10, 2013

Related Experiment Videos

Last Updated: Jun 21, 2026

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
10:06

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Published on: November 13, 2011

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
07:01

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

Published on: April 11, 2015

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
13:32

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Published on: April 10, 2013

Area of Science:

  • Molecular Biology
  • Genomics
  • Histology

Background:

  • Laser capture microdissection (LCM) isolates specific cells from tissue sections for gene expression analysis.
  • Obtaining high-quality RNA from limited starting material is a significant challenge in LCM studies.

Purpose of the Study:

  • To develop a simple, flexible, and cost-effective method for RNA isolation from LCM-dissected embryonic mouse brain cells.
  • To optimize critical steps in the LCM and RNA extraction procedure.

Main Methods:

  • Optimized staining (1% cresyl violet in 70% ethanol) and cryosectioning (20 µm thick).
  • Standardized storage of sections and harvesting of microdissected tissue in RNA stabilization solution.
  • Incorporated three flexible stop-points into the protocol.

Main Results:

  • Consistently obtained high-quality RNA (RNA integrity numbers > 8) from four simultaneously microdissected cell groups.
  • Successfully amplified long cDNA fragments (> 1.2 kb) via reverse transcription PCR.
  • Demonstrated method's flexibility without compromising RNA quality.

Conclusions:

  • The optimized LCM protocol yields high-quality RNA suitable for downstream applications.
  • Enables robust gene expression profiling in discrete cell populations using quantitative PCR or microarrays.
  • Provides a reliable method for researchers working with limited biological samples.