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Skin Tattooing As A Novel Approach For DNA Vaccine Delivery
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Published on: October 18, 2012

DNA tattoo vaccination: effect on plasmid purity and transfection efficiency of different topoisoforms

S G L Quaak1, J H van den Berg, K Oosterhuis

  • 1Department of Pharmacy & Pharmacology, Slotervaart Hospital/The Netherlands Cancer Institute, Louwesweg 6, 1066 EC Amsterdam, The Netherlands. Susanne.Quaak@slz.nl

Journal of Controlled Release : Official Journal of the Controlled Release Society
|July 8, 2009
PubMed

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...