Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...
Fluid Mosaic Model01:19

Fluid Mosaic Model

Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich with the analogy of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Insulin enables acquisition of the IL7R<sup>+</sup> memory phenotype in PD1<sup>+</sup> T cells in RA tissues.

Cell death & disease·2026
Same author

A user-friendly goniometer-compatible fixed-target platform for macromolecular crystallography at synchrotrons.

Journal of applied crystallography·2026
Same author

Free fatty acid receptor 2 allosterism is defined by cellular context.

Cell communication and signaling : CCS·2026
Same author

Systematic position of the enigmatic <i>Mirlatia arcuata</i> moth resolved: a monotypic tribe within the basal branches of Larentiinae (Lepidoptera, Geometridae).

ZooKeys·2026
Same author

Time-resolved X-ray solution scattering observations of light-induced structural changes in sensory rhodopsin II.

Structure (London, England : 1993)·2026
Same author

Time-resolved x-ray solution scattering from detergent solubilized visual rhodopsin.

Biophysical journal·2026
Same journal

Progress toward linking single-molecule behavior and condensate material properties.

Current opinion in structural biology·2026
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
See all related articles

Related Experiment Video

Updated: Jun 21, 2026

Crystallization of Membrane Proteins in Lipidic Mesophases
11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

Membrane protein crystallization from lipidic phases.

Linda C Johansson1, Annemarie B Wöhri, Gergely Katona

  • 1Department of Chemistry, Biochemistry and Biophysics, University of Gothenburg, SE-405 30 Gothenburg, Sweden.

Current Opinion in Structural Biology
|July 8, 2009
PubMed
Summary
This summary is machine-generated.

Lipidic phase crystallization is a powerful complementary technique for membrane protein structural biology, enabling the determination of structures resistant to traditional methods. This approach enhances protein stability within a lipid matrix, leading to successful crystallization and high-resolution structures.

More Related Videos

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

From Constructs to Crystals &#8211; Towards Structure Determination of &#946;-barrel Outer Membrane Proteins
09:55

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

Related Experiment Videos

Last Updated: Jun 21, 2026

Crystallization of Membrane Proteins in Lipidic Mesophases
11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

From Constructs to Crystals &#8211; Towards Structure Determination of &#946;-barrel Outer Membrane Proteins
09:55

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

Area of Science:

  • Membrane protein structural biology
  • Biophysics
  • Structural biology

Background:

  • Traditional crystallization methods face challenges with many membrane protein targets.
  • Lipidic phase crystallization offers a complementary approach for difficult-to-crystallize proteins.

Purpose of the Study:

  • To highlight the emerging role and impact of lipidic phase crystallization methods.
  • To discuss the advantages of lipidic environments for membrane protein stability and crystallization.

Main Methods:

  • Lipidic cubic phase, lipidic sponge phase, and bicelle crystallization methods.
  • Immersion of purified membrane proteins within a lipid-rich matrix.
  • Direct crystallization of detergent-solubilized proteins (traditional method for comparison).

Main Results:

  • Lipidic phase crystallization has yielded high-resolution structures of key membrane proteins.
  • Examples include beta(2)-adrenergic G-protein-coupled receptor, A(2A) adenosine G-protein-coupled receptor, and mitochondrial voltage dependent anion channel.
  • The lipidic environment is hypothesized to enhance protein stability, favoring crystallization.

Conclusions:

  • Lipidic phase crystallization is a powerful complement to traditional methods in membrane protein structural biology.
  • Technical innovations are making these methods more accessible.
  • The impact of lipidic phase crystallization is expected to increase with further development.