Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A H<sub>2</sub>@C<sub>60</sub><sup>1</sup>H NMR probe for sensitively detecting the supramolecular interactions between a molecular tweezer and C<sub>60</sub>.

Chemical communications (Cambridge, England)·2024

Same author

<sup>19</sup>F-NMR Unveils the Ligand-Induced Conformation of a Catalytically Inactive Twisted Homodimer of tRNA-Guanine Transglycosylase.

ACS chemical biology·2022

Same author

DeerLab: a comprehensive software package for analyzing dipolar electron paramagnetic resonance spectroscopy data.

Magnetic resonance (Gottingen, Germany)·2021

Same author

Resolving distance variations by single-molecule FRET and EPR spectroscopy using rotamer libraries.

Biophysical journal·2021

Same author

Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase.

Nature communications·2021

Same author

Unraveling a Ligand-Induced Twist of a Homodimeric Enzyme by Pulsed Electron-Electron Double Resonance.

Angewandte Chemie (International ed. in English)·2021

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Published on: September 1, 2020

Cryogenic 35GHz pulse ENDOR probehead accommodating large sample sizes: Performance and applications.

René Tschaggelar¹, Besnik Kasumaj, Maria Grazia Santangelo

¹Laboratory of Physical Chemistry, ETH Zurich, Zürich CH-8093, Switzerland.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)

|July 8, 2009

Summary

A new cryogenic 35GHz pulse electron nuclear double resonance (ENDOR) probehead for large samples was constructed. This probehead enhances experimental efficiency and applicability for paramagnetic samples containing transition metal ions.

More Related Videos

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Published on: February 25, 2021

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Published on: October 13, 2014

Related Experiment Videos

Last Updated: Jun 21, 2026

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Published on: September 1, 2020

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Published on: February 25, 2021

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Published on: October 13, 2014

Area of Science:

Physics
Chemistry
Spectroscopy

Background:

Electron Nuclear Double Resonance (ENDOR) is a powerful technique for studying paramagnetic species.
Cryogenic probeheads are essential for maintaining sample stability and improving signal-to-noise ratios at low temperatures.
Previous ENDOR probeheads often had limitations regarding sample size, restricting certain applications.

Purpose of the Study:

To present the construction and performance of a novel cryogenic 35GHz pulse ENDOR probehead.
To enable the study of larger sample volumes, thereby increasing sensitivity and applicability.
To investigate the electromagnetic field distributions within the probehead for optimized performance.

Main Methods:

Design and construction of a rectangular TE(102) cavity resonator.
Integration of a two-turn saddle coil for radio frequency (rf) B(2)-field generation.
Electromagnetic field calculations including all dielectric components, sample tube, and coupling elements.
Characterization using an rf power efficiency factor over a 2-180MHz frequency range.

Main Results:

Successful construction and characterization of the cryogenic 35GHz pulse ENDOR probehead.
Detailed analysis of microwave B(1)- and E(1)-field, and rf B(2)-field distributions.
Demonstration of the probehead's applicability and advantages for large sample sizes.
Validation using paramagnetic samples containing transition metal ions.

Conclusions:

The developed cryogenic 35GHz pulse ENDOR probehead is effective for large samples.
The design allows for efficient generation of the rf B(2)-field.
This advancement broadens the scope of ENDOR studies, particularly for transition metal ion-containing samples.