Related Experiment Video
Updated: Jun 21, 2026

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
Published on: August 18, 2017
Visualization of enantiomers using natural abundant (13)C-filtered single and double quantum selective refocusing
Nilamoni Nath1, Bikash Baishya, N Suryaprakash
1NMR Research Centre, Indian Institute of Science, Bangalore 560012, India.
Abstract:
The routine use of proton NMR for the visualization of enantiomers, aligned in the chiral liquid crystal solvent poly-gamma-benzyl-l-glutamate (PBLG), is restricted due to severe loss of resolution arising from large number of pair wise interaction of nuclear spins. In the present study, we have designed two experimental techniques for their visualization utilizing the natural abundance (13)C edited selective refocusing of single quantum (CH-SERF) and double quantum (CH-DQSERF) coherences. The methods achieve chiral discrimination and aid in the simultaneous determination of homonuclear couplings between active and passive spins and heteronuclear couplings between the excited protons and the participating (13)C spin. The CH-SERF also overcomes the problem of overlap of central transitions of the methyl selective refocusing (SERF) experiment resulting in better chiral discrimination. Theoretical description of the evolution of magnetization in both the sequences has been discussed using polarization operator formalism.
Related Concept Videos
¹H NMR Chemical Shift Equivalence: Enantiotopic and Diastereotopic Protons
In chiral compounds such as 2-butanol, replacing the methylene hydrogens at C3 produces a pair of...
Properties of Enantiomers and Optical Activity
Racemic Mixtures and the Resolution of Enantiomers
Chirality in Nature
Molecules with Multiple Chiral Centers
Prochirality

