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Updated: Jun 21, 2026

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
Published on: June 6, 2016
Retrogenes in preimplantation embryo development: a unique mode of transcriptional regulation
Chiu-Jung Huang1, Kong-Bung Choo
1Department of Animal Science, School of Agriculture, Chinese Culture University, Taipei, Taiwan, ROC.
Abstract:
Our studies show that retrogenes are preferentially expressed in preimplantation embryos. These genes carry a short noncoding exon 1 that contributes directly to expression of the gene, and a second exon that contains the coding sequence without intron interruption. We show that preimplantation gene expression is first regulated by developmentally regulated transcription factors that target exon 1 and the solitary intron, followed by promoter hypermethylation on implantation and in adult tissues. An understanding of the mechanisms of gene expression during preimplantation development should have an impact on the understanding and treatment of spontaneous abortion and infertility.
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