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Related Concept Videos

Activation of Integrins01:15

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
Integrins01:10

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

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Related Experiment Video

Updated: Jun 21, 2026

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
14:23

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Published on: August 27, 2012

Quantitative proteomics identifies a Dab2/integrin module regulating cell migration.

Anjali Teckchandani1, Natalie Toida, Jake Goodchild

  • 1Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

The Journal of Cell Biology
|July 8, 2009
PubMed
Summary

Dab2 (Disabled-2) protein regulates cell migration by internalizing cell surface integrins. This maintains an intracellular pool of integrins for new cell adhesions.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Clathrin-associated endocytic adapters are crucial for initiating endocytosis by recruiting cargo molecules.
  • Dab2 is an endocytic adapter protein involved in cellular processes.

Purpose of the Study:

  • To identify glycoprotein cargoes of the endocytic adapter Dab2 using a quantitative proteomics approach.
  • To investigate the role of Dab2 in regulating cell migration and integrin dynamics.

Main Methods:

  • Unbiased quantitative proteomics to identify Dab2 cargo.
  • Cellular assays using Dab2-deficient HeLa cells.
  • Analysis of integrin surface levels, localization, and cell migration speed.

Main Results:

  • Dab2 deficiency led to increased surface levels of specific integrin chains (beta1, alpha1, alpha2, alpha3).
  • Dab2 colocalized with integrin beta1 in coated pits, indicating its role in integrin endocytosis.
  • Dab2 depletion inhibited cell migration and polarized movement of integrin beta1 and vinculin.

Conclusions:

  • Dab2 mediates the internalization of cell surface integrins, particularly inactive ones.
  • Dab2 regulates cell migration by maintaining an intracellular pool of integrins for recycling to the leading edge.