Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 21, 2026

Kupffer Cell Isolation for Nanoparticle Toxicity Testing
09:49

Kupffer Cell Isolation for Nanoparticle Toxicity Testing

Published on: August 18, 2015

Evaluation of nanoparticle immunotoxicity.

Marina A Dobrovolskaia1, Dori R Germolec, James L Weaver

  • 1Nanotechnology Characterization Laboratory, SAIC-Frederick Inc., NCI-Frederick, Frederick, Maryland 21702, USA. marina@mail.nih.gov

Nature Nanotechnology
|July 8, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Validation of the Electrophilic Allergen Screening Assay for Detection of Key Event 1 of the Skin Sensitization Adverse Outcome Pathway.

Toxics·2026
Same author

Immune system evaluation of Echinacea Purpurea root extract in female Harlan Sprague Dawley (Hsd:Sprague Dawley SD) rats.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2026
Same author

Assessment of immunotoxicity in the 21st century: Where we are and what we need to replace animals.

Current opinion in toxicology·2026
Same author

RNA-DNA Fusomer Fibers With Customizable Physicochemical, Mechanical, and Biological Properties for Next-Generation Therapeutics.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Activation of an adaptive antitumor immune response by the polymeric fluoropyrimidine CF10 involves TS/Top1 dual targeting.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

When small gets smart: how the application of nanotechnology is transforming immunology in the 21<sup>st</sup> Century.

Nanomedicine (London, England)·2026

Evaluating nanoparticle drug immune responses is challenging. This review offers strategies to identify and control interferences in common testing methods for pharmaceutical development and regulation.

Area of Science:

  • Pharmaceutical Science
  • Immunotoxicology
  • Nanotechnology

Background:

  • The pharmaceutical industry increasingly utilizes nanoparticle-based drugs and diagnostics.
  • Evaluating the immune response to these novel formulations presents significant challenges for researchers and regulatory bodies.
  • Current testing methodologies have limitations in accurately assessing nanoparticle immunogenicity.

Purpose of the Study:

  • To address the challenges in evaluating immune responses to nanoparticle-based pharmaceuticals.
  • To identify and propose strategies for controlling interferences in common immunotoxicity assays.
  • To discuss the regulatory implications of nanoparticle formulation assessment.

Main Methods:

  • Convened an international expert panel workshop at the National Cancer Institute.

More Related Videos

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity
08:37

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

Published on: October 25, 2016

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
06:15

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Published on: January 30, 2019

Related Experiment Videos

Last Updated: Jun 21, 2026

Kupffer Cell Isolation for Nanoparticle Toxicity Testing
09:49

Kupffer Cell Isolation for Nanoparticle Toxicity Testing

Published on: August 18, 2015

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity
08:37

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

Published on: October 25, 2016

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
06:15

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Published on: January 30, 2019

  • Reviewed existing methods for evaluating immune responses to nanoparticles.
  • Identified common sources of interference in these assays.

    • Main Results:

    • Current assays for nanoparticle immunogenicity suffer from limitations and interferences.
    • Practical strategies for identifying and mitigating assay interferences were developed.
    • The implications of these findings for regulatory evaluation were considered.

    Conclusions:

    • Standardized and validated methods are crucial for assessing nanoparticle drug safety.
    • Addressing assay interferences is essential for reliable immunotoxicity evaluation.
    • Improved testing strategies will facilitate the regulatory approval of nanoparticle-based therapeutics.