Related Experiment Video
Updated: Jun 21, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
Two-point-separation in a sub-micron nonscanning IR super-resolution microscope based on transient fluorescence
Keiichi Inoue1, Nándor Bokor, Satoshi Kogure
1Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Yokohama 226-8503, Japan.
Abstract:
IR absorption of chemical species in microscopic objects such as biological cells cannot be measured by conventional IR microscopes, because of their low resolution. To overcome this problem, we developed a novel far-field IR super-resolution microscope employing transient fluorescence detected IR spectroscopy. The resolution of this microscope was shown to be 880 nm by measuring the image of 1 microm fluorescent beads. Furthermore, it succeeded in resolving beads located 1.4 microm apart from each other. This is considerably smaller than the diffraction limit of the applied IR light (3.4 microm). These results suggest the capability of our microscope to study sub-micron targets such as sub-cellular structures of biological cells.
More Related Videos
Related Concept Videos
Super-resolution Fluorescence Microscopy
Total Internal Reflection Fluorescence Microscopy
Confocal Fluorescence Microscopy

