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Related Experiment Video

Updated: Jun 21, 2026

Paradigms for Behavioral Assessment in Drosophila Model of Autism Spectrum Disorder

Paradigms for Behavioral Assessment in Drosophila Model of Autism Spectrum Disorder

Published on: September 6, 2024

Association study between BDNF gene polymorphisms and autism by three-dimensional gel-based microarray.

Lu Cheng¹, Qinyu Ge², Pengfeng Xiao¹

¹State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.

International Journal of Molecular Sciences

|July 8, 2009

Summary

This summary is machine-generated.

Brain-Derived Neurotrophic Factor (BDNF) gene variations are linked to autism susceptibility. A novel microarray method efficiently identified significant differences in BDNF C270T polymorphism and haplotypes in autism patients.

Keywords:

DNA microarray autism brain-derived neurotrophic factor (BDNF)single nucleotide polymorphism (SNP)

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A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia

A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia

Published on: June 15, 2011

Area of Science:

Genetics
Neuroscience
Biotechnology

Background:

Single nucleotide polymorphisms (SNPs) are crucial genetic markers for identifying disease susceptibility genes.
Complex diseases require high-throughput SNP genotyping methods for large-scale association studies.

Purpose of the Study:

To investigate the association between four Brain-Derived Neurotrophic Factor (BDNF) gene polymorphisms and autism.
To evaluate a polyacrylamide gel-based microarray with dual-color hybridization as a high-throughput SNP genotyping method.

Main Methods:

Applied polyacrylamide gel-based microarray with dual-color hybridization for SNP genotyping.
Analyzed four BDNF polymorphisms in autism patients and control groups.
Conducted allele, genotype, and haplotype association analyses.

Main Results:

The C270T polymorphism in BDNF showed significant differences in allele and genotype frequencies between autism patients and controls.
Global haplotype distribution analysis revealed significant differences between the groups.
The microarray method proved rapid, simple, and high-throughput for SNP genotyping.

Conclusions:

BDNF gene polymorphisms, particularly C270T, may play a role in the pathogenesis of autism.
The developed microarray technique is effective for large-scale association studies of genetic susceptibility in complex disorders.