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Related Experiment Videos

Thymic nuclear matrix associated activity is not V(D)J recombinase.

V N Pandey1, V P Dave, S B Amrute

  • 1Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.

Biochemical and Biophysical Research Communications
|November 27, 1991
PubMed
Summary

Nuclear matrix from young rat and fetal calf thymus shows recombination activity. However, analysis indicates this activity does not result from V(D)J signal sequence recombination.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Previous studies reported nuclear matrix from young rat thymus supports V(D)J recombination.
  • Nuclear matrix is a structural component of the cell nucleus.

Purpose of the Study:

  • To investigate the recombination activity detected in nuclear matrix from fetal calf thymus.
  • To determine if the observed activity is due to V(D)J signal sequence recombination.

Main Methods:

  • Isolation of nuclear matrix from fetal calf thymus.
  • Treatment of plasmid substrates with nuclear matrix.
  • Restriction enzyme mapping of recombined plasmid products.

Main Results:

  • Nuclear matrix from fetal calf thymus exhibited recombination-supporting activity.

Related Experiment Videos

  • Restriction enzyme mapping revealed the double antibiotic resistance was not caused by V(D)J signal sequence recombination.

    • Conclusions:

    • The observed activity in the nuclear matrix does not stem from V(D)J signal sequence recombination.
    • Further investigation is needed to elucidate the mechanism of the observed recombination activity.