Related Experiment Video
Updated: Jun 21, 2026

A Technical Guide for Performing Spectroscopic Measurements on Metal-Organic Frameworks
Published on: April 28, 2023
Solvent effect on the UV/Vis absorption and fluorescence spectroscopic properties of berberine
Marta Susana Díaz1, Mónica Liliana Freile, María Isela Gutiérrez
1Departamento de Química, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, 9000, Comodoro Rivadavia, Argentina.
Abstract:
Spectral and photophysical properties of the alkaloid berberine (B) were studied in solvents with different solvent parameters, using UV/Vis absorption, emission and excitation spectroscopy. The absorption and emission maxima were found to be between 421-431 nm and 514-555 nm, respectively, leading to Stokes' shifts between 4099 and 5735 cm(-1). The fluorescence quantum yields varied between 10(-2)-10(-4), depending on the solvent. Different solvent scales have been used to study the solvatochromism of B. Linear solvation energy relationships (LSER) proposed by Kamlet-Taft suggest that B is a molecule attractive as a probe for solvent polarity and hydrogen bonding properties.
Related Concept Videos
UV–Vis Spectrum
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...
UV–Vis Spectroscopy of Conjugated Systems
One of the factors influencing λmax is the extent of conjugation in the...
UV–Vis Spectrometers
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
UV–Vis Spectroscopy: Beer–Lambert Law
UV–Vis Spectroscopy: Molecular Electronic Transitions
