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Updated: Jun 21, 2026

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
07:16

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Published on: March 14, 2014

Methodologies in assaying prostate cancer stem cells.

Hangwen Li1, Ming Jiang, Sofia Honorio

  • 1Department of Carcinogenesis, University of Texas MD Anderson Cancer Center, Smithville, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 8, 2009
PubMed
Summary
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Cancer stem cells (CSCs) drive tumor growth. This review details methods for identifying CSCs in prostate cancer (PCa) using in vitro assays, xenografts, and primary samples.

Area of Science:

  • Oncology
  • Cancer Biology
  • Stem Cell Research

Background:

  • The cancer stem cell (CSC) theory suggests a small subpopulation of tumor cells drives tumor initiation, homeostasis, and progression.
  • Tumorigenic potential and self-renewal are key characteristics defining CSCs, observed in various cancers.
  • Identifying and characterizing CSCs is crucial for understanding cancer development and devising effective therapies.

Purpose of the Study:

  • To comprehensively review experimental methodologies for assaying putative prostate cancer stem cells (PCa SCs).
  • To present the methods utilized by the lab for evaluating PCa SCs in different contexts.
  • To provide a framework for identifying and validating CSC properties in prostate cancer.

Main Methods:

  • Assaying single-cell tumorigenic potential in foreign microenvironments (xenografts).

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Last Updated: Jun 21, 2026

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
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Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Published on: March 14, 2014

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
09:06

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Published on: December 13, 2019

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
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  • Performing in vitro assays to demonstrate stem cell-associated biological properties.
  • Analyzing CSCs within cultured cells, xenograft tumors, and primary tumor samples.

    • Main Results:

    • The review details established and validated methods for PCa SC identification.
    • Experimental approaches cover in vitro, in vivo (xenograft), and primary sample analyses.
    • The methodologies aim to rigorously test the CSC properties of prostate cancer cell populations.

    Conclusions:

    • Accurate identification of CSCs requires a multi-faceted approach combining in vitro and in vivo assays.
    • The presented methodologies provide a robust framework for studying PCa SCs.
    • Understanding CSCs is essential for developing targeted therapies to eradicate prostate cancer.