Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA

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Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

Marian A C Piqueur1, Walter A Verstrepen, Peggy Bruynseels

  • 1Department of Microbiology, ZNA Hospitals, site Middelheim, Lindendreef 1, 2020 Antwerp, Belgium. marianpiqueur@hotmail.com

Virology Journal
|July 9, 2009
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...