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Updated: Jun 21, 2026
An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach
Published on: December 7, 2016
Vitreous concentration of triamcinolone acetonide after a single transseptal depot injection
Paolo S Silva1, Ravinder J Singh, Sophie J Bakri
1Sentro Oftalmologico Jose Rizal, University of the Philippines-Philippine General Hospital, Manila, Philippines.
Purpose:
To determine vitreous concentrations of triamcinolone acetonide(TA) following transseptal injection.
Methods:
TA (40 mg/l ml) was administered at varying intervals prior to vitrectomy. Vitreous samples from 18 patients were obtained. TA concentrations were quantified using HPLC-MS.
Results:
Vitreous levels of TA were detected in all samples (range 0.64-168.0 ng/ml). The interval from injection to sampling ranged from 0.5-20 days. Tmax was attained at 0.5 days. TA concentrations were negatively correlated with scleral thickness (P = 0.0184) and depot location (P = 0.0230) but not with PVD status (P = 0.9633).
Conclusion:
Significant vitreous concentrations of TA are achieved after a single transseptal injection. Scleral thickness, depot location and time influence vitreous TA levels.
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