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Updated: Jun 21, 2026

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
Published on: August 14, 2018
The use of self-interaction chromatography in stable formulation and crystallization of proteins
Kedar S Deshpande1, Tangir Ahamed, Joop H ter Horst
1Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Abstract:
This paper reviews the basic principles of the recently developed self-interaction chromatography (SIC) technique with regard to protein solution stability and protein crystallization. It gives experimental protocols for both normal-scale and micro-scale SIC experiments and reviews recent developments and current applications of this novel technique in the biopharmaceutical area. This paper aims to be a benchmark in the further proliferation of this highly effective and fast technology for the rational design of stable aqueous formulations of therapeutic proteins and the determination of solution conditions favoring protein crystallization.
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