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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: Jun 21, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

[A new SNP microarray technology based on single base extension].

Yuan-Yuan Dong1, Hai-Hui Sheng, Qian Yang

  • 1National Engineering Center for Biochip at Shanghai, Shanghai 201203, China. dongyuanyuan_dyy@yahoo.com.cn

Yi Chuan = Hereditas
|July 10, 2009
PubMed
Summary
This summary is machine-generated.

This review summarizes biochemical methods for studying DNA-protein interactions in vivo and in vitro. It covers traditional assays and modern chromatin immunoprecipitation techniques for epigenetic research.

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Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

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Last Updated: Jun 21, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
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Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
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Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Context:

  • DNA-protein interactions are crucial for fundamental biological processes.
  • Understanding these interactions is key to deciphering cellular mechanisms.

Purpose:

  • To review various biochemical methods for studying DNA-protein interactions.
  • To highlight both traditional and advanced techniques.

Summary:

  • The article discusses in vivo and in vitro methods at the DNA, protein, and complex levels.
  • Traditional assays like Nitrocellulose filter-binding assay, Footprinting, Electrophoretic Mobility Shift Assay (EMSA), and Southwestern blotting are summarized.
  • Advanced chromatin immunoprecipitation (ChIP) techniques, including native ChIP (nChIP), crosslinked ChIP (xChIP), and ChIP-on-chip, are introduced for epigenetic studies.

Impact:

  • Provides a comprehensive overview of essential techniques for researchers.
  • Facilitates the study of gene regulation and epigenetic modifications.
  • Aids in understanding the molecular basis of various life processes.