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Related Experiment Video

Updated: Jun 11, 2026

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
12:39

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

Published on: June 24, 2011

BSAC standardized disc susceptibility testing method (version 8).

J M Andrews1,

  • 1Department of Microbiology, Sandwell and West Birmingham NHS Trust, City Hospital, Birmingham B18 7QH, UK. jenny.andrews@swbh.nhs.uk

The Journal of Antimicrobial Chemotherapy
|July 10, 2009
PubMed
Summary
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This update revises antibiotic susceptibility testing guidelines, simplifying interpretation and enhancing accuracy for various bacterial infections. Key changes include revised breakpoints and streamlined recommendations for urinary tract infections.

Area of Science:

  • Microbiology
  • Clinical Pharmacology
  • Infectious Diseases

Background:

  • Previous antibiotic susceptibility testing recommendations (version 7) required significant updates to improve clarity and accuracy.
  • Ambiguities in interpreting results, particularly for urinary tract infections (UTIs) and multi-site infections, necessitated a format revision.
  • The organization of antibiotic recommendations into distinct groups (e.g., beta-lactams, aminoglycosides) aimed to reduce confusion.

Purpose of the Study:

  • To present revised antibiotic susceptibility testing breakpoints and recommendations based on updated scientific evidence.
  • To enhance the interpretation of antimicrobial susceptibility testing (AST) results for a wide range of bacterial pathogens.
  • To streamline guidelines for treating common infections, particularly UTIs, by refining the application of intermediate susceptibility categories.

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Published on: September 9, 2015

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
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Published on: August 11, 2018

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Last Updated: Jun 11, 2026

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
12:39

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

Published on: June 24, 2011

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
11:28

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

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A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
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A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

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Main Methods:

  • Systematic review and analysis of current antimicrobial susceptibility data.
  • Establishment of revised minimum inhibitory concentration (MIC) and zone diameter breakpoints (BPs) for numerous antibiotic-pathogen combinations.
  • Modification of recommendation formats, including the relocation of footnotes and the specific handling of UTI-related breakpoints.

Main Results:

  • Significant updates to MIC and zone diameter breakpoints for key antibiotics including trimethoprim, fosfomycin, nitrofurantoin, doripenem, colistin, co-trimoxazole, and others.
  • Revised recommendations for specific pathogens such as Pseudomonas spp., Stenotrophomonas maltophilia, staphylococci, Streptococcus pneumoniae, enterococci, and Neisseria species.
  • Streamlined UTI recommendations, with intermediate susceptibility now explicitly applicable for UTI interpretation when systemic recommendations exist.

Conclusions:

  • The revised guidelines offer a clearer and more accurate framework for antibiotic susceptibility testing.
  • Updated breakpoints and simplified recommendations are expected to reduce interpretation errors and improve clinical decision-making.
  • These changes facilitate optimized antimicrobial therapy selection for a broad spectrum of bacterial infections.