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Related Concept Videos

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Updated: Jun 21, 2026

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
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Acidic-store depletion is required for human platelet aggregation.

Nidhal Ben Amor1, Hanene Zbidi, Aicha Bouaziz

  • 1Unité de Recherche de Biochimie et Interaction Moléculaire, Institut Supérieur de Biotechnologie, 5019-Monastir, Tunisia.

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|July 10, 2009
PubMed
Summary

Acidic calcium stores are crucial for human platelet aggregation. Inhibiting these stores significantly reduces thrombin-induced aggregation, highlighting their functional role.

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Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
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Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

Area of Science:

  • Hematology
  • Cell Biology
  • Biochemistry

Background:

  • Human platelets utilize intracellular calcium stores for aggregation.
  • Two primary stores exist: the dense tubular system and lysosomal-like acidic stores.
  • The specific role of acidic stores in platelet aggregation requires further elucidation.

Purpose of the Study:

  • To investigate the contribution of lysosomal-like acidic calcium stores to thrombin-induced platelet aggregation.
  • To determine if depleting acidic stores affects platelet aggregation mediated by protease-activated receptors (PARs) and GPIb-IX-V.

Main Methods:

  • Platelet aggregation assays were performed in Ca(2+)-free and Ca(2+)-containing media.
  • The sarcoendoplasmic Ca(2+)-ATPase (SERCA)3 selective inhibitor, 2,5-di-(tert-butyl)-1,4-hydroquinone, was used to discharge acidic Ca(2+) stores.
  • Aggregation was measured following stimulation with thrombin, PAR-1 agonist (SFLLRN), PAR-4 agonist (AYPGKF), and GPIb-IX-V activation.

Main Results:

  • Thrombin-induced platelet aggregation was reduced in a Ca(2+)-free medium.
  • Treatment with the SERCA3 inhibitor significantly reduced thrombin-evoked platelet aggregation.
  • Depletion of acidic stores diminished aggregation induced by PAR-1, PAR-4 agonists, and GPIb-IX-V activation.

Conclusions:

  • Ca(2+) accumulation in acidic compartments is essential for platelet aggregation.
  • Acidic Ca(2+) stores play a functional role in thrombin receptor (PAR-1, PAR-4) and GPIb-IX-V-mediated platelet aggregation.
  • These findings support the functional significance of lysosomal-like acidic Ca(2+) stores in human platelets.