Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Long COVID symptoms before and 3-35 months after SARS-CoV-2 infection in a Norwegian prospective cohort study, symptoms over the pandemic, and different virus variants.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026

Same author

Acute COVID-19 severity and impaired cognitive function up to 32 months after diagnosis: an observational study.

BMC medicine·2026

Same author

Multimodal epigenetic and enhancer network remodeling shape the transcriptional landscape of human beige adipocytes.

Communications biology·2026

Same author

Major waves of H2A.Z incorporation during mouse oogenesis and preimplantation embryo development.

Nature communications·2025

Same author

Broad H3K4me3 domains support oocyte genome silencing and maturation but are dispensable for repression in early embryos.

Development (Cambridge, England)·2025

Same author

Loss of multilevel 3D genome organization during breast cancer progression.

Genome research·2025

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Published on: August 29, 2017

MicroChIP: chromatin immunoprecipitation for small cell numbers.

John Arne Dahl¹, Philippe Collas

¹Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.

Methods in Molecular Biology (Clifton, N.J.)

|July 10, 2009

Summary

This study introduces a rapid micro (micro)ChIP assay for analyzing protein-DNA interactions using minimal cell numbers. This fast chromatin immunoprecipitation technique enables multiple parallel assays from just 1,000 cells.

More Related Videos

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Published on: May 10, 2019

Related Experiment Videos

Last Updated: Jun 21, 2026

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Published on: August 29, 2017

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Published on: May 10, 2019

Area of Science:

Molecular Biology
Genomics
Epigenetics

Background:

Chromatin immunoprecipitation (ChIP) is crucial for studying protein-DNA interactions and mapping histone modifications.
Conventional ChIP requires large cell numbers, limiting its use with rare cell samples.
Existing ChIP assays for small cell numbers are often time-consuming.

Purpose of the Study:

To develop a fast and efficient ChIP assay for small cell populations.
To enable multiple parallel ChIP analyses from a single chromatin batch.
To optimize ChIP for immunoprecipitating histone proteins and transcription factors.

Main Methods:

Development of a rapid micro (micro)ChIP assay.
Application to small cell numbers ranging from 100 to 100,000 cells.
Performed under cross-linking conditions for histone proteins and transcription factors.

Main Results:

The microChIP assay is suitable for multiple parallel ChIPs from 1,000 cells.
A single immunoprecipitation can be performed using as few as 100 cells.
The assay significantly reduces the time and cell input required for ChIP.

Conclusions:

The rapid microChIP assay provides a streamlined method for studying protein-DNA interactions in limited cell samples.
This technique expands the applicability of ChIP to rare cell types and small-scale experiments.
The assay facilitates high-throughput epigenomic studies with reduced sample requirements.