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Updated: Jun 21, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Applications of ultra-high-throughput sequencing.

Samuel Fox¹, Sergei Filichkin, Todd C Mockler

¹Department of Botany and Plant Pathology and Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 10, 2009

Summary

Ultra-high-throughput sequencing (UHTS) revolutionizes biological research by enabling genome-wide studies at unprecedented speed and reduced cost. This technology accelerates discovery in genomics, transcriptomics, and biotechnology, impacting our fundamental understanding of life.

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Area of Science:

Genomics and Molecular Biology
Plant Science and Biotechnology

Background:

The genomics era allows global questions on mutation, evolution, and gene regulation.
Sanger sequencing enabled molecular basis studies, but ultra-high-throughput sequencing (UHTS) offers greater advancements.

Purpose of the Study:

To provide an overview of ultra-high-throughput sequencing (UHTS) approaches and applications.
To describe a protocol for deep sequencing of plant transcriptomes using the Illumina/Solexa platform.

Main Methods:

Utilizing ultra-high-throughput DNA sequencing technologies.
Applying UHTS for genome sequencing, resequencing, small RNA discovery, SNP discovery, ChIP-seq, RIP-seq, transcriptome analysis, alternative splicing discovery, and gene expression profiling.
Developing and describing a deep sequencing protocol for plant transcriptomes.

Main Results:

UHTS provides vast amounts of DNA sequence data rapidly, cost-effectively, and with reduced effort compared to Sanger sequencing.
Applications span comparative genomics, systems biology, metagenomics, and genome biology.
The technology significantly impacts plant breeding, biotechnology, and understanding plant evolution, development, and environmental responses.

Conclusions:

UHTS is transforming biological research by enabling comprehensive, genome-wide analyses.
Its applications are vast and limited only by researcher creativity.
This technology is crucial for advancing plant science and biotechnology.